《植物生理学报》 2017, 53(3): 352-362

生物安全型选择标记基因及其应用研究进展

田野, 柴薇薇, 包爱科, 王锁民^*

兰州大学草地农业科技学院, 草地农业生态系统国家重点实验室, 兰州730020

通信作者：王锁民；E-mail: smwang@lzu.edu.cn

摘　要：

在植物基因工程中, 选择标记基因(selectable marker genes, SMGs)的应用使“真”转化子得以辨认, 但目前常用的抗生素及除草剂类SMGs可能对人类及家畜健康、生态平衡等带来潜在的危害, 这也是影响转基因植物商业化的最重要因素之一。鉴于此, 基于可视化、非生物胁迫、代谢关键酶的安全SMGs迅速崛起, 并成功用于转化子筛选, 且筛选精度与效率明显提高。本文旨在对近年来生物安全型SMGs的有效性及适用性的研究进展进行分析与综述, 以期为转基因植物的广泛推广奠定基础。

关键词：转基因植物; 安全标记; 生物安全性

收稿：2016-12-23   修定：2017-01-21

资助：国家自然科学基金(31470503)、教育部博士点基金优先发展领域项目(20130211130001)和甘肃省科技支撑计划项目(144FKCA0558)。

Research progress of biosafety selectable marker genes

TIAN Ye, CHAI Wei-Wei, BAO Ai-Ke, WANG Suo-Min^*

State Key Laboratory of Grassland Agroecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China

Corresponding author: WANG Suo-Min; E-mail: smwang@lzu.edu.cn

Abstract:

In genetic engineering, selectable marker genes (SMGs) are generally deployed to identify ‘true’ transformants. The frequently-used SMGs such as antibiotics or herbicides generally obtained from prokaryotes, posing risks due to their lethal nature. In this regard, some safety SMGs based on visual, abiotic stress and metabolism are more attractiveand successfully used for screening of transformants with high selection efficiency. This review mainly focus on their availability and applicable range which contributes to breeding biosafety transgenic plants.

Key words: transgenic plants; safety maker; biosafety